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How to Create Feminized Cannabis Seeds. Reference Article Lubell, J. D., & Brand, M. H. (2018). Foliar sprays of silver thiosulfate produce male flowers on female hemp plants. HortTechnology, 28(6), 743-747. https://journals.ashs.org/horttech/view/journals/horttech/28/6/article-p743.xml Basics of Creating Feminized Seed To produce all female seed requires induction of female plants to develop male flowers that produce pollen that will be genetically female. Female sex chromosomes: XX Male sex chromosomes: XY So when a female plant (XX) is induced to form pollen, since there is no “Y” chromosome all of the resulting pollen will be XX (female). When pollinated with a known female plant (also XX) this will result in the offspring being female (XX). How To Induce Male Flowers on a Female Plant? 3mM Silver Thiosulfate (STS) applied as a foliar spray (on three occasions 7 days apart) to produce male flowers The 3mM STS treatment was shown on four strains of female hemp (having a THC concentration of ≤0.3%), designated CBD hemp A, CBD hemp B, CBD hemp C, and industrial hemp. Study Specifics (Materials and Methods) Rooted cuttings were about 6 inches high with two shoots when foliar sprays of STS were initiated. Silver thiosulfate solutions (0.3 and 3 mM) were prepared by mixing silver nitrate with STS in a molar ratio of 1:4, per Cameron and Reid (1981). *See end of slide show for exact preparation steps of 3 mM Silver thiosulfate solution Plants were sprayed to runoff (about 20 mL/plant) on three occasions, 7 days apart, beginning on 30 Apr. 2018 for the first replication and on 14 May 2018 for the second replication of this study. Control plants received sprays of water only. On 30 Apr. and 14 May, plants began receiving short-day conditions of about 8 h using a blackout curtain to initiate the flowering stage. Study Results Between 14 and 21 days after the first STS spray treatment, plants transitioned from first flowers to full flowering. The most complete inflorescence conversion to male flowers occurred at 3 mM STS for all hemp strains. At 3 mM STS, the majority of inflorescences had 100% male flowers and the remaining inflorescences had 75% male flowers. Untreated control plants of all four hemp strains produced only female flowers, as expected Reduced Pollen Production Male flowers that developed on genetically female plants produced pollen. However, the pollen did not appear to be as plentiful or freely released as observed on true genetically male plants. The pollen did produce seed, which was harvested, germinated, and exposed to short-day conditions. The resulting plants were confirmed to be female. Possible Explanation for Mode of Action of STS Ethylene blocking by STS is likely responsible for the production of male flowers on female hemp plants in our study. It is generally believed that ethylene blocking is extended when a series of sprays of STS is used compared with a single spray of STS (Reid et al., 1980). It may be possible to induce male flowers on genetically female hemp using other ethylene perception inhibiting chemicals such as 1- methylcyclopropene. Conclusion This study demonstrates that male flowers can be produced easily and consistently on female plants through application of foliar sprays of STS under short-day conditions. Overall, STS at 3 mM was the most effective treatment for producing the greatest number of male flowers on female hemp plants. we did not observe any plant phytotoxicity or delay in flower development. Furthermore, we were able to achieve 95% to 100% conversion to male flowers for all hemp strains. Preparation of 3 mM Silver Thiosulfate Preparation Of Silver Thiosulfate (STS) Solution Link to Lecture Slides: https://drive.google.com/file/d/1JDoJpVq4dY_bLyqbzBwHUpPZe0MI-67Y/view?usp=sharing 420 Magazine Source Content by Slide Title: How To Induce Male Flowers https://www.420magazine.com/community/gallery/190628-sts-tools-png.1691501/ Study Specifics https://www.420magazine.com/community/gallery/12-2-sts-6.134449/ https://www.420magazine.com/community/gallery/12-2-sts-3.134446/ Reduced Pollen Production https://www.420magazine.com/community/gallery/img_6612-jpg.1784797/ https://www.420magazine.com/community/gallery/sp-pollen.1771970/ Preparation https://www.420magazine.com/community/gallery/silver-thiosulfate-solution.1244950/ *Due to the description character limit the full work cited for "How to Create Feminized Cannabis Seeds" can be viewed at... https://drive.google.com/file/d/1PeUlymPM8Unej4v3F6ZTbJrNAaYdOfT2/view?usp=sharing

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Dr. Jason Busse describes the latest research findings regarding some potential benefits of medicinal cannabis. Recorded as a part of the Innovations in the Science of Cannabis Conference, October 2023. In this lecture, Dr. Jason Busse, Associate Director of the Michael G. DeGroote Center for Medicinal Cannabis Research, delves into the therapeutic potential of cannabis for chronic pain and other medical conditions. With over 380,000 Canadians authorized to use medical cannabis, Dr. Busse explores the evolving landscape of cannabis use, from its legalization for medical purposes in 2001 to its widespread availability today. He discusses the growing body of evidence supporting cannabis for conditions like pediatric seizures, multiple sclerosis, and chronic pain, while also addressing the risks and limitations of current research. Dr. Busse highlights the importance of patient values and preferences in guiding treatment decisions and shares findings from systematic reviews and clinical trials. Whether you're a healthcare professional, patient, or simply curious about cannabis as a therapeutic option, this presentation offers a balanced, evidence-based perspective on its benefits and challenges. _______________________________________________________ Medical cannabis benefits Cannabis for chronic pain Therapeutic uses of cannabis CBD vs THC for pain relief Cannabis legalization in Canada Cannabis for multiple sclerosis Cannabis and opioid reduction Risks of medical cannabis Cannabis for sleep disorders Cannabis research updates _______________________________________________________ The Michael G. DeGroote Centre for Medicinal Cannabis Research is a multidisciplinary leader in medicinal cannabis research. The Centre is led by the Michael G. DeGroote School of Medicine Initiative for Innovation in Healthcare at McMaster University in Hamilton, Ontario, Canada. This initiative leverages McMaster University’s world-renowned expertise in health research and evidence-based medicine to create a hub for evidence-based data and innovative research. https://cannabisresearch.mcmaster.ca/

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